Rapid and simple method for the determination of emodin in tartary buckwheat (Fagopyrum tataricum) by high-performance liquid chromatography coupled to a diode array detector[J].

Peng L X, Wang J B, Hu L X, et al. Journal of Agricultural & Food Chemistry, 2013, 61(4):854-7.

ABSTRACT: A simple and rapid method for determining emodin, an active factor presented in tartary buckwheat (Fagopyrum tataricum), by high-performance liquid chromatography coupled to a diode array detector (HPLC-DAD) has been developed.Emodin was separated from an extract of buckwheat on a Kromasil-ODS C18 (250 mm × 4.6 mm × 5 μm) column. The separation is achieved within 15 min on the ODS column. Emodin can be quantified using an external standard method detecting at 436 nm. Good linearity is obtained with a correlation coefficient exceeding 0.9992. The limit of detection and the limit of quantification are 5.7 and 19 μg/L, respectively. This method shows good reproducibility for the quantification of the emodin with a relative standard deviation value of 4.3%. Under optimized extraction conditions, the recovery of emodin was calculated as>90%. The validated method is successfully applied to quantify the emodin in tartary buckwheat and its products.
KEYWORDS: Emodin, tartary buckwheat, HPLC-DAD, quantification